在生物科研、农产品及种子检验和进出口商品检验检疫等领域,经常需要对将植物组织进行研磨以获得DNA、RNA或蛋白质等生物大分子,从而进行后续的分析和检测。随着实验室科研进度的日益加快,手动研磨已无法满足科研工作的需要,越来越多的科研人员都开始使用植物组织研磨器进行研磨,不仅省时省力,也更简单高效。其中,目前比较普及的研磨机器大都基于研磨钢珠高速碰撞原理,最常见的主要有2大类,一类是净信快速组织研磨仪,代表机型如某公司生产的Tissuelyser/JX-FSTPRP系列的自动组织研磨器,另一类则是常规的研磨机器,比较有代表性的是德国某品牌组织研磨机。那么这2类机器有哪些性能特点呢?在使用上有无区别?研磨效果方面有哪些差异?来自实验的您,又该如何选择机型呢?本文以上面2种代表机型做参考进行比较,对这些问题做一个简要总结:
那么如何选择适合自己的机型呢?
选择常规研磨机理由:需要一次研磨处理大于200个微量样品,或者一次处理超大体积样品时;不需要冷冻研磨。
选择净信快速组织研磨仪理由:习惯在实验室台面上操作;一次处理量在200个微量样品以下,同时需要研磨较大体积或质地坚硬的样品;需要经常进行冷冻研磨;
关于价格方面,就目前了解到的信息,净信快速植物组织研磨仪与国产常规的研磨机器的价格相差不大(后者均价略贵),而两种都有不同型号配置的机器,所以定价不一,但市场价位一般在3-6万左右,相比同类进口机型动辄10万以上的高价,实则优惠较多。实际上,无论技术与质量,目前国产和进口研磨机器的差距已经非常小,而在现货以及售后方面,国产研磨机器则具有明显优势,毕竟省去了漫长的货期和高昂的维修费是广大用户喜闻乐见的。而出于爱国主义情怀,笔者当下也更倾向于选择国产机器。当然,最终选择净信快速组织研磨仪还是常规组织研磨器必须结合实验室的实际需求与具体预算综合考虑了。
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