一、研究原理
果实发育及表观遗传学:番茄是世界范围内的重要蔬菜作物,同时也因为其较小的基因组,完整的遗传图谱以及较短的生命周期被作为研究果实生长成熟的模式植物。对于番茄果实成熟的研究不仅能帮助了解果实成熟的机制,也对提高番茄果实品质,以及其他肉质果实品质具有重要的作用。近年来,番茄的果实成熟被越来越广泛深入地研究,果实成熟的调控机制也越来越清晰。 最新研究显示,DNA甲基化可能是转录因子和植物激素之外的第三个最重要的调节果实成熟的因子。然而,DNA甲基化参与调控果实成熟的机制则缺乏相应的深入研究。
二、实验地点:中国科学院上海植物逆境生物研究中心朗瞾博课题组
三、研磨方法
实验步骤:
1,准备好待研磨的番茄叶片样品几个, 半导体适配器,液氮,清洗并灭菌;
2,将样品置于钢罐中,同时将直径的研磨钢珠放入钢罐中,将其整体侵入液氮几分钟之后并安装到上海净信研磨仪上;
3,设置频率,时间,开机进行研磨;
4,待机子停止运行,取出研磨钢罐。得出的实验结果如下:
研磨对比
Previous:冷冻研磨仪JXFSTPRP-CL研磨番茄的实验方法
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