人和动物的肝脏具有极强的再生能力,研究人员也通常用大鼠来做实验从中去研究肝脏的再生能力。通过分离、纯化再生肝中各种细胞,研究大鼠肝再生肝脏细胞增殖周期变化及体外细胞增殖周期。
大鼠肝70%切除后可在7-10天内基本恢复原有的体积和功能。从负调机制角度对肝再生进行研究,阐明调控肝再生终止和肝脏组织结构重塑的分子机制,不仅有助于全面认识肝脏再生,同时对肝脏疾病,尤其是肝脏肿瘤病理机制的认识和治疗靶点的探索等都具有十分重要的意义。
杭州阿诺生物医药科技有限公司在样品前处理这块也在使用我们净信研磨仪,这次他们的实验对象是大鼠。
实验地点:阿诺医药
实验材料和器具:
样品:大鼠浸泡保存的脏器
仪器:全自动快速研磨仪(上海净信JX-24)
配件:24金属适配器、氧化锆珠、氧化锆珠
JXFSTPRP?系列研磨机采用了特殊的上下及左右晃动三维一体的震动模式,样品在空间呈8字形三维运动,通过研磨珠(氧化锆、钢珠、玻璃珠、陶瓷珠)的高频往复振动、撞击、剪切。快速的实现目的。使研磨的样品具有更加充分、更均匀、样品重复性更好、样品之间没有交叉污染。
实验步骤:
1、将整块的大鼠浸泡保存的脏器处理成黄豆大小以备研磨;
2、将样品加入到无酶离心管中;
3.、加入若干颗氧化锆珠或氧化锆珠;
4、设定运行参数
5、启动仪器至研磨程序结束,可得到如图所示样品。
Previous:净信高能研磨仪在茶研究中的应用
Next:全自动样品研磨仪---处理化工PE材料
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