6月,可谓是吃货的季节。尝不尽的各式鲜果,应季时蔬。这个时候便是玉米大量上市的季节,几乎家家户户的饭桌上都会出现它的身影。玉米中含有大量的营养物质,不仅含有碳水化合物、蛋白质,还有大量对人体有益的营养物质,深得人们的喜爱。
玉米全身都是宝,今天咱们来说说玉米壳纤维。吃完香甜的玉米,可别急着扔掉玉米壳,玉米壳可以变废为宝。它可以成为色彩丰富又环保的手工艺品,科学家们也可以使用玉米壳生产廉价的氢燃料成为再生燃料。
东华大学张彦中教授也在利用玉米壳来研究仿生材料与再生医学,张老师一直从事纤维生物材料与人体组织器官再生的基础和应用研究。通过合理调控纤维生物材料的化学、生物、结构、力学等要素来模拟(干)细胞生存的微环境,从而增强(干)细胞对生物材料支架的生物响应特性和对伤病组织的再生修复能力。
此次张教授的实验目的是根据实验要求需把玉米壳纤维研磨碎进行分析和检测,随后根据实验要求做后续实验
实验材料和器具:
样品:玉米壳纤维
仪器:研磨仪(上海净信,JXFSTPRP-48)
配件:聚乙烯研磨罐,配套研磨珠.
耗材:液氮
JXFSTPRP系列研磨仪采用了特殊的上下及左右晃动三维一体的震动模式,样品在空间呈8字形三维运动,通过研磨珠(氧化锆、钢珠、玻璃珠、陶瓷珠)的高频往复振动、撞击、剪切??焖俚氖迪帜康?。使研磨的样品具有更加充分、更均匀、样品重复性更好、样品之间没有交叉污染
产品特点:
1.操作数量多,高效快速效果好抽提的蛋白比活更高,核酸片断更长。
2.无交叉污染,操作简便,重复性好
3.稳定性好噪音小于55dB,不会对其它实验或仪器产生干扰。
4.方便低温操作
实验步骤:
1.准备好若干ml聚乙烯研磨罐,配套研磨珠。
2.把准备好的玉米纤维放入聚乙烯研磨罐中放入配套研磨珠盖好,放入液氮中浸泡若干分钟;
3.把侵泡好的罐子放入冷冻研磨仪中,设置参数,程序结束后即可破碎。
5.完成后做后续实验。
6.进行分析再做再生实验。
实验结果:
注意事项:
1.研磨罐和研磨珠需充分清洗干净并烘干备用;则研磨罐和研磨珠要确保灭酶;
2.每个研磨罐研磨样品量一般遵循原则:样品体积不超过离心管容积的三分之一。
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