脐带是哺乳类的连接胎儿和胎盘的管状结构,由两条动脉和一条静脉构成。脐带呈圆柱状结构,内有两条脐动脉及一条脐静脉。脐带内有一条脐静脉,接受从母体来的新鲜血液并流经胎儿全身回到胎儿的动脉系统,最后进入两条脐动脉流入胎盘至母体,这是胎儿血循环的特点之一。胎儿藉以生存的营养物质如电解质、无机盐、激素、微量元素、免疫抗体等,均需经过脐带传送。
脐带也一直是科研人员竞相研究的一块领域,例如脐带间充质干细胞,它联合造血干细胞移植可发挥强大的造血支持能力。科学家发现间充质干细胞具有调节免疫的作用,有着广泛的临床应用前景。它可以改善骨髓造血微环境,促进造血干细胞的植入,显著加快移植后的造血重建,安全且并发症少。同时,适量的间充质干细胞在造血干细胞移植中,可以降低移植物抗宿主病的发生率和严重程度。
山东银丰生物集团就利用上海净信浸入式液氮冷冻研磨仪研磨脐带,提取RNA。净信便携式液氮冷冻研磨仪功能强大,是世界上技术领先的液氮冷冻研磨设备,全过程超低温运行,是市场上公认的高效的研磨设备:其冷冻研磨方法是一种先进的、高效的、方便的样品处理方法,可以处理常规方法难以处理的样品,如:塑料、橡胶、树脂、皮革、毛发、骨骼、木材、纺织原料等等,被称为研磨的终极手段。液氮冷冻研磨仪应用领域广泛,如生物化学中的RNA/DNA检测、聚合物结构分析、痕迹元素的判断、食品分析、矿物质研究、医疗外科手术的辅助、药品性能的检测、纺织品组成成分的鉴定等等。
冷冻组织研磨仪
实验目的:通过研磨脐带组织,提取RNA
实验材料和器具:
样品:脐带组织
工具:净信全自动样品快速研磨仪
耗材:专用研磨罐,液氮、剪刀、镊子等
实验步骤:
1、准备研磨罐和样品;
2、取组织剪成小块放入研磨罐中,将盖子盖紧;
3、将研磨罐置于装有液氮的容器内,液氮容量没过研磨罐冷冻几分钟,取出时戴手套或使用镊子夹取避免液氮冻伤;
4、首先将适配器放入研磨仪中固定,然后在将研磨罐放置适合器内,以保证研磨时的受力平衡,旋紧垂直定位螺旋,将安全锁扣紧。
5、设置参数启动程序结束后即可得到破碎粉末;
6、研磨完毕后请关闭电源,将安全锁归位;
7、完成后续RNA提取步骤。
实验解决的问题:
1、仪器研磨相对手工研磨速度更快,缩短整体实验时间;
2、仪器研磨出料颗粒均匀、细腻,无需过筛,可直接进行下一步提取工作;
3、避免样品污染与浪费
实验过程、结果图示:
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact