大鼠(RAT),学名Rattus norvegicus,在生物分类学上属脊椎动物门、哺乳动物纲、啮齿目、鼠科、家鼠属、褐家鼠种。实验室也常会看到大鼠的影子,大鼠在药物学、代谢性疾病研究、肿瘤研究遗传学等多个研究领域做出了很大的贡献。此次试验的目的是研磨大鼠血管,提取核酸。
实验材料和器具:
样品:大鼠血管
工具:净信多样品组织研磨仪(Tissuelyser-24)
耗材:离心管、研磨珠、液氮、剪刀、镊子等
实验步骤:
1、准备离心管,研磨珠若干;
2、首先将适配器置于装有液氮的容器内,液氮容量没过适配器即可,冷冻几分钟,取出时戴手套或使用镊子夹取避免液氮冻伤;
3、从冰箱内取大鼠血管组织剪成小块与研磨珠一同放入离心管中,将盖子盖紧;
4、将适配器放入研磨仪中固定,然后在将离心管放置适合器内。样品尽量对称放置,以保证研磨时的受力平衡,旋紧垂直定位螺旋,将安全锁扣紧。设置参数,程序结束后即可得到破碎粉末;
5、研磨完毕后请关闭电源,将安全锁归位;
6、完成后续核酸提取步骤。
注意事项:
1、每支离心管样品量一般不超过放心管体积的三分之二,样品量过多会影响研磨效果。
2、样品尽量对称放置,以保证研磨时的受力平衡。
3、适配器采用半导体材质需要提前放入液氮中浸泡几分钟预冷。
实验过程、结果图示:
实用仪器介绍:
上海净信?的Tiss组织研磨仪是一种特殊的、快速的、高效率的、多试管的一致系统。它能将任何来源(包括土壤、植物和动物的组织/器官、细菌、酵母、真菌、孢子、古生物标本等)的原始DNA、RNA和蛋白质进行提取和纯化。
采用了特殊的垂直上下一体震动模式,通过研磨珠(氧化锆、钢珠、玻璃珠、陶瓷珠)的高频往复振动、撞击、剪切??焖俚氖迪帜康摹J寡心サ难肪哂懈映浞?、更均匀、样品重复性更好、样品之间没有交叉污染。
Previous:净信全自动样品快速研磨仪:研磨鱼骨提取微量元素
Related Products
- Home
- About us
-
Product
-
Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
-
Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
-
General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
-
Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
-
Vibrating?sieve?shakers
Vibrating?sieve?shakers
-
Foreign distribution products
Foreign distribution products
-
Grinder for scientific research
- Customers
- News
- Videos
- Case
- Cantact