实验目的:实验室里的老师需要用到研磨设备,来研磨植物组织提DNA(比如柑橘、柿子之类),偶尔需要研磨一些其他组织,由于之前没有接触过我们的这类产品,我们便和老师们一起做了此次的研磨实验。
实验地点:武汉大学
实验材料和器具:
样品:生柿子
工具:上海净信全自动样品研磨仪(Tissuelyser-24)
耗材:离心管,研磨珠(钢珠)
实验步骤:
1.准备好适配器,钢珠
2.将装好的样品规范放入Tissuelyser-24研磨仪中
批量研磨样品,一般情况下,由于客户研磨的是生柿子,较难处理,纤维较多,果肉较硬每个研磨管放若干颗4mm钢珠,设置频率,若需要研磨更细致可以加钢珠
3.得到如图所示研磨样品
注意:
1、每支离心管样品量一般不超过放心管体积的三分之二。
2、样品尽量对称放置,以保证研磨时的受力平衡。
实验过程、结果图示:
上海净信Tissuelyser-24
如图为我们的实验材料,研磨的是生柿子
实用仪器介绍:
此款仪器为上海净信实业发展有限公司生产的全新全自动样品研磨仪是一种特殊的、快速的、高效率的、多试管的一致系统。它采用了特殊的垂直上下一体震动模式,通过研磨珠(氧化锆、钢珠、玻璃珠、陶瓷珠)的高频往复振动、撞击、剪切。快速的实现目的。使研磨的样品具有更加充分、更均匀、样品重复性更好、样品之间没有交叉污染。
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