客户介绍:浙江农林大学位于杭州市临安区、是浙江省重点建设高校、浙江省人民政府与国家林业和草原局共建高校。学校以农林、生物、环境学科为特色,涵盖八大学科门类的多科性大学。
实验目的:愈伤组织(callus)是指原植物体的局部受到创伤刺激后,在伤口表面新生的组织。它由活的薄壁细胞组成,可起源于植物体任何器官内各种组织的活细胞。本次实验对象为愈伤组织,要求磨细,而且冻液氮磨,后续用来提RNA。
实验步骤:
1、准备JXFSTPRP-24L组织研磨仪一台、离心管、氧化锆研磨珠以及所需研磨愈伤样品若干。
2、取适量愈伤组织、一颗氧化锆研磨珠一起放入离心管中,盖子盖上。
3、将配备好的离心管放入液氮中预冷,冻脆样品。金属适配器底座也一起放入液氮预冷(预冷2-3分钟)
4、将离心管适配器放入研磨仪中固定(适配器底部凹槽与轴的卡槽互相卡牢)放平后将离心管分别放入适配器对应孔位,(注意平面配平,对称放样)盖上适配器盖,旋紧螺帽。
5、设置参数(根据所需研磨量微调时间),开始研磨。
6、磨好后打开仪器取出离心管,即可进行后续实验。
(愈伤组织) (装管图)
(研磨效果图) (取样图)
上图为1分钟的研磨效果,已经完全研磨完成。效果非常好,老师要求愈伤研磨一定要冻液氮,否则rna可能降解,所以人工研磨特别不方便,经过多次了解和对比所以老师们选用净信研磨仪代替人工处理。效率高,效果好。完美解决了老师的研磨问题。
注意事项:
1、液氮操作需佩戴操作液氮的厚橡胶手套,注意安全。
2、放研磨管时时注意平面配平,只有一个样品时需用空管配平。
3、冻液氮过程中一定是装好样品和珠子关盖的研磨管,不要开盖浸入液氮,液氮发热膨胀容易爆管。
实验选用仪器: 全自动样品快速研磨仪 JXFSTPRP-24L
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