实验目的:关于土壤中所含微生物细胞内容物的提取。
实验地点: 浙江省农业科学院土肥所。
实验材料和器具:
样品:干燥土壤粉末或颗粒
仪器:全自动样品快速研磨仪(上海净信)、金属适配器
耗材:土壤中微生物细胞内容物提取试剂盒(试剂、配好研磨珠的离心管)
实验步骤
1、称量干燥土壤粉末或颗粒;
2、将称量好的样品加入到试剂盒所配离心管中(图1);
3.、先加入试剂,然后加入裂解液在涡旋仪上初步混匀;
4、设定好运行参数,将样品管放入适配器中;
5、启动仪器至研磨程序结束,可得到研磨后样品(图2);
6、将样品放入离心机中几分钟后(图3),吸取上清液进仪器分析
实验结果:
图1:样品加入后;图2:净信研磨仪研磨后;图3:离心过后待提上清
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