蘑菇是由菌丝体和子实体两部分组成,菌丝体是营养器官,子实体是繁殖器官,它是真菌中的一种,可分为:食用蘑菇、药用蘑菇、毒蘑菇。蘑菇的种类非常多,我们今天要讲的是双孢蘑菇的质量检测......
1、课题组介绍:
农业部食用菌产品质量监督检验测试中心(上海)常务副主任和技术负责人邢增涛关于双孢蘑菇的研究。
农业部食用菌产品质量监督检验测试中心(上海),研究条件重点开展蔬菜、水果、食用菌、粮油产品质量安全检测、农产品质量安全风险评估、农产品质量安全风险控制与预警、农产品安全保鲜等方面的研究。
2、实现的目的:
根据实验要求需把双孢蘑菇进行研磨以获得DNA、RNA或蛋白质等生物分子,从而进行后续的分析和检测。随着实验室科研进度的日益加快,手动研磨已无法满足科研工作的需要,故而选择上海净信全自动冷冻研磨仪进行样品研磨。
3、实验准备
实验地点:实验室
样品:双孢蘑菇
仪器:全自动冷冻研磨仪(上海净信,JXFSTPRP-CL)
配件:聚乙烯研磨罐,配套研磨珠.
耗材:液氮
JXFSTPRP-CL(专利产品,仿冒必究!ZL2017305264903)样品处理的三大关键要素:
○高效率研磨——一批处理多达192个样品
○低温研磨——研磨温度可自定义调节
○不破坏样品成分——低温保护样品成分
产品应用:
·提取核酸/蛋白质,低温研磨样品能够有效抑制核酸降解、保留蛋白质活性。
·分析药效成分,药物有效成分的异构体之间常常存在重大区别。低温研磨可预防分子因压力和受热而降解。
·减少样品挥发,低温研磨能显著减少样品的挥发,更完整保留样品成分含量
·研磨韧性/硬性样品,对于高韧塑料、高强塑料、树脂等难研磨样品,低温研磨可大大提高研磨效果及效率
4、实验步骤:
1.准备好聚乙烯研磨罐;配套研磨珠.
2.把准备好的双孢蘑菇放入聚乙烯研磨罐中放入配套研磨珠盖好,放入液氮中浸泡若干分钟;
3.把装双孢蘑菇的罐子侵泡后,取出放入冷冻研磨仪中,设置参数启动,程序结束后即可破碎为粉末状。
5.研磨完成后继续做后续实验。
研磨对比图:
5、注意事项:
1、研磨罐和研磨珠需充分清洗干净并烘干备用;则研磨罐和研磨珠要确保灭酶;
2.每个研磨罐研磨样品量一般遵循原则:样品体积不超过离心管容积的三分之一。
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Orbital shaker
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Standard vacuum freeze dryer
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Vertical mixer
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Non-contact ultrasonic DNA interrupter
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Rotary mixer HS-5
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Non-contact shaped DNA interrupter
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Orbital shaker
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Roller mixer (blood mixer)
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Dual-channel non-contact energy-concentrated DNA interrupter
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Rotational viscometer
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Cup type ultrasonic cell crusher
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Inching vortex oscillator
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Handheld ultrasonic cell pulverizer
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Handheld ultrasonic cell pulverizer
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Ultra-micro ultrasonic cell crusher
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Multifunctional vortex oscillator
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Rotational viscometer
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Handheld ultrasonic cell crusher
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Micro centrifuge
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Integrated ultrasonic cell crusher
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Handheld ultrasonic cell crusher
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Multi-tube vortex oscillator
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Rotational viscometer
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Micro centrifuge
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Ultrasonic crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher integrated
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Microplate centrifuge
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Snowflake ice maker
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Integrated ultrasonic cell crusher
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Ultrasonic crusher
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Nitrogen Blowing Apparatus
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Ultrasonic crusher
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Split ultrasonic cell disruptor
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Microplate horizontal centrifuge
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Snowflake ice maker
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Ultrasonic cell crusher
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Integrated ultrasonic cell crusher
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Nitrogen Blowing Apparatus
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Round water bath nitrogen blowing instrument
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Ultrasonic cell crusher
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Dual-channel non-contact energy-concentrated DNA interrupter
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Nitrogen Blowing Apparatus
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Ultrasonic cell crusher
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Visual nitrogen blowing instrument
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Intelligent ultrasonic crusher
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Ultrasonic cell crusher
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Ultrasonic crusher
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Constant temperature airtight ultrasonic reactor
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Non-contact ultrasonic pulverizer
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Automatic sample fast grinder-24L
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Soil Ball Mill
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Microwave ultrasonic extractor
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Soil Ball Mill
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Automatic sample fast grinder-32L
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Planetary Ball Mill
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Planetary Ball Mill
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Planetary Ball Mill
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Automatic sample fast grinder-48L
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High energy grinder
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High-throughput soil grinder
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Glass bead sterilizer
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Automatic sample fast grinder-64L
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Automatic sample fast grinder-96L
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Infrared inoculation loop sterilizer
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Hair drug detector
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Infrared inoculation loop sterilizer
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Hair screening drug detector
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Hair treatment instrument
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Portable hair grinder
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Biological indicator incubator
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Hair Grinding and Shredder
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Multi-sample tissue grinding machine-FEIII
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Multi-sample tissue grinder-FEII
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Tissue grinder
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Tissue grinding machine
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Plant tissue processing system
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Multi-sample tissue grinding machine-192
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Multi-sample tissue grinder-96
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Multi-sample tissue grinder-64
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Multi-sample tissue grinder -48
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Multi-sample tissue grinding machine-32
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Multi-sample tissue grinder-24
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QuEChERS pre-processing all-in-one machine
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QuEChERS sample preparation system
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流体实验鲁尔转接头
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细胞定位格子培养皿
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Thermostatic metal bath
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μ-Dish 35 mm, high Grid-50 Glass Bottom
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Thermostatic metal bath
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Thermostatic metal bath
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Mini refrigerated metal bath
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Mini thermostat metal bath
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六通道载玻片
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Thermostatic metal bath refrigeration type
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μ-Dish 35 mm, high tissue culture dish
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In situ hybridization
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8孔腔室载玻片
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Multifunctional cell electric fusion instrument
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UV crosslinker
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细胞定位格子培养皿
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12孔可移除腔室载玻片
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六通道载玻片0.1
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鲁尔固定连接器-F
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鲁尔固定接头-M
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鲁尔公接头
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鲁尔公插头
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Molecular hybridization furnace
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CultureWell?可重复使用硅胶垫片
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内毒素检测
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TH-5热敏性温度监测仪
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BAT-12微探针温度计
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BAT-10 多功能温度计
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灌注管道装置
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灌注槽
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灌注设备
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2孔腔室载玻片-无凹液面
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18孔培养载玻片-扁平版
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API Coryne
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常规培养基
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CoverWell?成像小室
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成像垫片(imaging spacer)
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一体化成像系统 (具备AI人工智能技术)
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High temperature four-module thermostatic metal bath
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荧光蛋白激发观察系统
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荧光蛋白激发观察系统
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便携式显微镜-带XY工作台
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PM-1 便携式显微镜
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细胞拉伸系统
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Culture Well?可移除腔室盖玻片
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其他设备(电生理/IVF)
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SecureSlip?带硅胶层盖玻片
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活细胞培养系统
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Three-dimensional mixer
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磁石培养皿
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CoverWell?灌流小室
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CultureWell?多孔腔室盖玻片
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共培养载玻片
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磁座组合式活细胞培养槽
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3D Centrifugal Cryogenic Grinder
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Small bead mill/cell bead mill
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Automatic sample rapid grinder-192
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Cryogenic Grinder JXFSTPRP-CLN
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Cryogenic tissue grinder
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Cryogenic tissue grinder
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High-speed cryogenic grinding homogenizer
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Multi-sample tissue grinder-24L
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High-speed tissue grinding machine KZ-II
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Multi-channel microbial magnetic bead homogenizer
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Automatic sample rapid grinding machine
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Automatic sample rapid grinder-24
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Immersion liquid nitrogen Cryogenic Grinder
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Automatic liquid nitrogen freezing grinding machine
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Automatic sample rapid grinding machine-64
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Automatic sample rapid grinding machine-48
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Constant temperature and humidity weighing system
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Vertical oscillator
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Multi-sample tissue grinder-192L
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Multi-sample tissue grinder-96L
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Multi-sample tissue grinder-64L
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Cryogenic Grinder (Semi-automatic Liquid Nitrogen Cryogenic Grinder)
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Multi-sample tissue grinder-32L
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3D sample tissue grinder
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Automatic sample rapid grinder-32
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Automatic sample rapid grinding machine
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Fully automatic sample rapid cryogenic grinder
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Fast tissue low-temperature crushing homogenizer
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Cryogenic Grinding Machine
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QuEChERS pre-processing all-in-one machine
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Multi-sample tissue grinder-48L
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96孔板
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High energy ball mill
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3D Cryogenic Grinder
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3D Centrifugal Cryogenic Grinder
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Cryogenic tissue grinder
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Frozen grinder
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Cryogenic grinder
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Basic cryogenic grinder
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Frozen Bone Teeth Crusher
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Mini grinder
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Mini Diamond Grinding Machine
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MINI rapid detection grinder
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Portable sample grinder
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Portable sample grinder
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Automatic sample rapid grinder-96
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Chlorophyll A grinder
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Semi-automatic hammer cyclone mill
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Automatic hammer cyclone mill
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Low temperature hammer type cyclone mill
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Multifunctional homogenizer
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Three-head shock type high-energy ball mill
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琼脂糖
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Water quality chlorophyll grinder
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Three-dimensional mixer
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Mortar grinder
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High-efficiency vibrating disc grinder
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Fully automatic cryogenic sample processing system
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Automatic sample rapid grinder
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Three-dimensional mixer
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Hot-lid metal bath
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Three-dimensional high-speed vibration ball mill
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NTE-2A温度探针&控制器
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Automatic homogenizer
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Beating sample homogenizer
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Solid phase extraction device
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Solid Phase Extraction Apparatus
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组织切片全系统
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High temperature type CNC super constant temperature bath
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Vibratron II振动型整流器
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Automatic rotating sampler
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Aquatic product animal husbandry solid phase extraction device
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Knife grinder
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培养皿载玻片保湿盒
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玻璃底培养皿
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串联连接管
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Biological indicator incubator
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弹性底部培养皿
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Beating aseptic homogenizer
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4孔腔室载玻片-无凹液面
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Oil-free vacuum pump
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WF-1内置沉淀过滤器
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细胞分离机附属冷冻系统
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TCAT-2 动物温度控制器
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MTC-1微型温度控制器
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定制热电偶
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加热毯WB-1, WB-2
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TCAT-2配件: 加热灯HL-1
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加热板HP-1M & HP-4M
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Cross-beating grinder
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交换器SWT-2
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Single cell suspension preparation instrument
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水泵和水箱装置
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TS-4MP恒温显微镜载物台
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Sample pre-processing machine
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Fully automatic homogenizer
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Beating aseptic homogenizer
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Grinding bead homogenizer
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Parallel grinder
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交换器SBT-5
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细胞趋化载玻片3D
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Jaw Crusher
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ibidi Pump System
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In-line Luer Injection Port
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μ-Plate Angiogenesis 96 Well
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μ-Slide y-shaped
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Constant temperature mixer (refrigeration)
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Constant temperature mixer
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μ-Slide I Luer Glass Bottom
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Culture-Insert 2 Well in μ-Dish 35 mm
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μ-Slide 8 Well high
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2D细胞趋化载玻片
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μ-Slide 15 Well 3D (formerly μ-Slide Angiogenesis)
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μ-Slide I
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μ-Dish Microscopy Rack
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sticky-Slide Chemotaxis
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Hose Clip
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Custom-Specific Flow Slides and Channels
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Dry thermostat
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减管径配件
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微4孔插件
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Single cell preparation instrument (temperature control)
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Microplate Shaker
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Microplate incubator
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Microplate incubator
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Oscillator special for microplate
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硅胶管
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μ-Slide 18 Well
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Microplate Heat Sealer
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器官运输器
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单通道载玻片
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三合一通道载玻片
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六通道平版载玻片
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单通道可粘载玻片
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六通道可粘载玻片
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Temperature-controlled single cell suspension preparation instrument
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μ-Slide I Luer 3D
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Microplate Oscillator
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梯度混匀器
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Ricordi? 注射袋
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High Power Handheld Grinder
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Bone and Teeth Grinder
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温度显示器
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温度探针
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温度探针延长线
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胰岛分离机
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Handheld Grinder
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分离管道装置
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Fully automatic low temperature flip oscillator
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API 20 E
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API 20 NE
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API 20 STAPH
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API 20 A
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API NH
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API Listeria
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API RapiD 20E
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Ricordi? 腔室系统
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Y型连接管
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无血清培养基
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细胞培养试剂
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原代细胞及推荐培养基
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支原体检测
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转染试剂
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8孔可粘腔室载玻片
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μ-Dish 35 mm, low
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New type soil drying box
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24孔板
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API ZYM/ API 50CHB/ API 50CHL
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2孔腔室载玻片
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4孔腔室载玻片
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Gradient amplification PCR detection analyzer THG-96G (gradient)
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Gene amplification instrument PCR instrument THT-96G (gradient type)
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High-speed grinding homogenizer-192
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Flip oscillator
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Fully Automatic Flip Oscillator
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DIC培养皿盖/载玻片盖
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胰岛计数皿
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High-throughput sample rapid grinder
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Medium Flux Grinder
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High Throughput Grinder
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High Throughput Tissue Grinder-2018
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High Throughput Tissue Grinder - GTL64
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氮化硅弹珠
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细胞抽样管
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菌种保存管
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胰岛细胞计数仪
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T型热电偶金属线
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生物流体灌流系统
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灌流小室
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灌流管道装置
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灌流喷头
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灌流腔室过滤器
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COBE? 2991 血细胞淘洗机
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闪胶
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BNC热电偶
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通用型探针
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胰岛运输器
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API Campy
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TH-8 热敏性检测温度计
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BAT7001H温度计
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THERMES USB 温度数据采集
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THERMES USB WFI 温度数据采集
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针型微探针
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Ultra High Throughput Tissue Grinder
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超细IT-系列灵活探针
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Ultra High Throughput Tissue Grinder
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临床探针
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动物直肠探针
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RET-2-ISO
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RET-3-ISO
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RET-4直肠探针
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IT系列特氟龙探针
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MT系列针型微探针
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API 10S
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灵活可植入探针
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Grinder for scientific research
Grinder for scientific research
- Enhanced multi-sample tissue grinder
- Enhanced automatic sample rapid grinder
- Enhanced cryomill
- Multi-sample tissue grinding machine
- Automatic sample rapid grinder
- Cryogenic tissue grinder
- Three-dimensional motion grinder
- Single cell suspension preparation instrument
- Freezer tissue grinder
- Liquid nitrogen Cryogenic Grinder
- High Throughput Tissue Grinder
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Grinder for analysis?and?detection
Grinder for analysis?and?detection
- Hair drug detector
- Handheld Grinder
- QuEChERS pre-processing all-in-one machine
- Soil/High Energy Planetary Grinder
- Hammer cyclone mill
- Knife grinder
- Multifunctional/Automatic Homogenizer
- Three dimensional vibrating ball mill
- Chlorophyll/soil A grinder
- Mortar grinder
- High-efficiency vibrating disc grinder
- Jaw grinder
- Flap homogenizer
- Bead-milling tissue grinder
- Special grinder for African swine fever
- Three-dimensional mixing instrument
- Miniature bead mill
- Forensic bone and tooth ball mill
- Percussion grinder
- Rotary splitter
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General?Instruments
General?Instruments
- Ultrasonic cell disruptor
- Ultrasonic equipment
- In situ hybridization instrument/n instrument/crosslinking
- instrument Dry type thermostat/constant temperature mixer
- Vortex mixer/nitrogen purging instrument
- PCR instrument/membrane sealing instrument Snow ice
- Microplate constant temperature shaking incubator
- Orbital shaker/micro centrifuge
- Inoculation loop sterilization/indicator incubator
- Solid phase extraction device/low temperature thermostat
- Snow ice maker/viscometer/lyophilizer
- Flip oscillator
- Soil drying box/humidity box
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Xiaomei Ultrasound Series
Xiaomei Ultrasound Series
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Vibrating?sieve?shakers
Vibrating?sieve?shakers
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Foreign distribution products
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Grinder for scientific research
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